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Expi293F™ human cells are derived from the 293 cell line, and are a core component of the Expi293™ Expression System. They are maintained in suspension culture and will grow to high density in Expi293™ Expression Medium. Expi293F™ cells are highly transfectable and generate superior protein yields compared to standard 293 cell lines in transient protein expression.
• Suspension culture cells pre-adapted to Expi293™ Expression Medium • Demonstrated higher protein expression levels than other 293 cells adapted to Expi293™ Expression Medium • Exhibit more rapid cell growth and higher culture viabilities than standard suspension-adapted 293 cells • Consistent transient expression performance of Expi293F™ cells for over 40 passages beyond thaw
Frozen cells are supplied in a vial containing 1 ml of cells at 1 x 107 viable cells/ml in Expi293™ Expression Medium and 10% DMSO. The cells should be thawed directly into Expi293™ Expression Medium. The six-vial multipack eliminates the need to expand and rebank the cells in the lab.
Cells are typically grown in flasks on a shaker platform in a humidified, CO2 cell culture incubator. However, cultures are scalable from volumes of <1 mL in multiwell plates to >10 L in bioreactors. Protein harvest can be performed at 2 to 7 days post-transfection, depending on the protein expressed. For expression of recombinant antibodies, harvest is typically at 5 to 7 days post-transfection. There is no need for culture medium supplementation or batch feeding during expression.
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