GIBCO® KnockOut™ CTS™ XenoFree ESC/iPSC Kit is a one kit solution for the preparation of serum-free, xeno-free media and growth factor supplements to perform feeder and feeder-free growth of human Embryonic Stem Cells (hESC) and human induced pluripotent stem cells (hiPSC). All GIBCO® CTS™ products are manufactured and quality controlled for reliable performance, and are supplied with harmonized regulatory documentation. This version includes components for creating 2.5 L of complete serum-free, xeno-free medium.

• High quality manufacturing and QC with access to traceability documentation (COA, COO, DMF)
• Feeder and feeder-free derivation and expansion without genome alteration
• Long-term passaging of your human ESC and human iPSC lines

Manufactured and Verified for Reliability and Simplified Transition to Clinical Studies
Each component in the KnockOut™ CTS™ XenoFree ESC/iPSC Kit is manufactured following cGMP and ISO 13485 guidelines to be fully defined and to contain only human-derived or human recombinant proteins to facilitate the transition of hESC research from the bench top to the clinic. Each lot is subject to QC bioassay to ensure lot to lot consistency and is supplied with harmonized documentation such as Certificates of Analysis (COA), Certificates of Origin (COO), and access to authorization letters for our Drug Master Files (DMF), as appropriate.

Simplified Complete Serum-Free, Xeno-Free Medium Formulation
KnockOut™ CTS™ XenoFree ESC/iPSC Kit contains everything needed to formulate 2.5 L of complete serum-free, xeno-free medium (animal component free). Purchasing this complete kit can save you time and money compared to purchasing growth factors and medias individually. However, if you don’t need the complete kit, each component can be purchased as a separate product:
Knockout™ SR GF Cocktail CTS™
Knockout™ SR XenoFree CTS™
KnockOut™ DMEM CTS™
bFGF CTS™

Derive, Expand, and Preserve Your Stem Cell Line with One Total Media
The KnockOut™ CTS™ XenoFree ESC/iPSC Kit can be used for hESC cryopreservation, hESC and induced pluripotent stem cell (iPSC) derivation, and routine culture maintenance. When combined, the components of this kit create a fully defined serum-free, xeno-free environment that can be used for long-term passaging of stem cell lines. This complete media supports multiple passages of human ESC lines such as BG01v and H9 under feeder-free conditions while retaining Oct 4 expression.

For Research Use or Non-Commercial Manufacturing of Cell-Based Products for Clinical Research.

CAUTION: Not intended for direct administration into humans or animals