TargetSeq™ Target Enrichment Kit is a solution-phase DNA probe capture technology that enables selective and specific enrichment of portions of a genome including an entire exome, or other customer-defined regions of interest, in a single tube. TargetSeq™ Target Enrichment Kits are conveniently packaged with all the reagents needed to perform target enrichment, including all hybridization and wash buffers, human cot-1 DNA, and Dynal M-270 streptavidin magnetic beads, with the exception of blocker oligonucleotides (TargetSeq™ Blocker Oligo Kits sold separately). TargetSeq™ kits enable enrichment of the whole exome or any target region between 0.1Mb and 60Mb in size and are offered in various kit configurations to meet most researcher’s needs. The TargetSeq™ Exome Enrichment product sets a new standard for exome sequencing by giving you the power to capture over 20,000 genes and sequence them on a single lane with a workflow that is optimized for short read sequencing.

Advantages of TargetSeq™ Exome Enrichment Kit

• Optimized target enrichment using a simple bead-based protocol for your 5500 Genetic Analyzer pipeline
• Integrated workflow with the AB Library Builder™ System for automation and LifeScope™ Genomic Analysis Solution for data analysis
• Cost-effective capture and robust multiplexing attained by pooling of up to 4 samples at the pre-enrichment step resulting in a price per sample cost as low as $125 (€99).
• Low DNA input - as low as 125ng of library per sample when performing multiplexed capture.

Optimized Target Enrichment with More Relevant Content
Proven turn-key solutions for targeted resequencing which meet rigid specifications and are economical on a per-sample cost basis. More relevant content including both predicted and validated genes covering >98% of Consensus Coding Sequences (CCDS) (37.3Mb Exon Target; 45.1Mb probe coverage). With in-depth development and optimization by the team at HGSC, Baylor College of Medicine, such specifications are achieved:

• Evenness in coverage as measured by % of reads covered at 10x and 20x depth.
• Number of SNPs detected at 10x and 20x coverage depths as compared to competitive products
• Percent of SNP concordance with dbSNP at 10x and 20x coverage depths as compared to competitive products

Integrated and Cost-Effective Capture Solution
TargetSeq™ enrichment products are optimized to provide exceptional and economical performance when combined with SOLiD® Libraries, the AB Library Builder™ automated library construction instrument, and SOLiD® 4 or 5500 Series Genetic Analysis Systems. TargetSeq™ Enrichment Kits are one of the first products to endorse multiplex sample capture (4-plex) using library bar-coding and pre-capture sample pooling which dramatically lowers the per sample costs and simplifies the workflow.

Low Sample Input Required for Efficient Exome Enrichment
TargetSeq Exome enrichment kits employ a multiplex capture protocol that requires as little as 125ng of each individual library, helping to conserve precious clinical research samples. The TargetSeq protocol also leverages bar-coded fragment libraries to enable fast, efficient, and economical sample enrichment prior to exome sequencing.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.