With the StemPro® CD34+ Cell Kit You Get:
- Human umbilical cord blood pooled from US donors to limit variability
- ≥90% purity of the desired CD34+ cell population
- StemPro-34 SFM for CD34+ cell expansion
- Media meeting full regulatory compliance – cGMP and ISO13485
- Full traceability documentation
- Publications and suggested protocols for multiple applications
- Application-specific testing to ensure lot consistency
- Availability in multiple lots
Hematopoietic progenitor cells (HPCs) or hematopoietic stem cells (HSCs), can be found in the blood, bone marrow, umbilical cord blood, or derived from Embryonic Stem Cells (ESCs) or induced pluripotent stem cells (iPSCs). They are multipotent stem cells capable of forming every cell lineage in the blood and immune system. Hematopoietic progenitor cells (HPCs) are non-adherent, with a small, round morphology. A fundamental property of these cells is their capacity to self-renew, thus providing, in principle, a limitless source of daughter cells with the same functional properties. They are most commonly used in transplant therapies to treat illnesses such as leukemia, lymphoma, and sickle cell diseases by reconstituting a patient’s hematopoietic cell system. HPCs/HSCs can also be reprogrammed to induced pluripotent stem cells (iPSCs) for use as disease models in stem cell research. Several cell surface markers used for identifying human HPCs/HSCs include CD34+, CD59+, Thy1/CD90+,CD38 lo/-, C-kit/CD117 +, lin-.
High Purity Stem Cells for Higher Reprogramming Efficiency
The most commonly used cell surface marker to define a human HPC/HSC population is the CD34 antigen. StemPro® CD34+ cells have ≥90% CD34+ cells, as determined by Flow Cytometry, affording higher reprogramming efficiency than less pure populations. To produce StemPro® CD34+ cells, cord blood from multiple human donors originating and collected in the United States is pooled, then an enriched CD34+ cell population is isolated using immunomagnetic CD34 MicroBeads. The use of United States donors increases control of cell origin, and pooling the cord blood from multiple donors reduces the lot-to-lot variability of the product. The cells are derived from tissue obtained from accredited institutions through an IRB (Institutional Review Board) approved donor program, and are negative for HIV and Hepatitis B. Each vial of cells contains 0.5 x 10e6 CD34+ cells in a volume of 1ml. Quality control for these cells includes testing for mycoplasma, sterility, endotoxin, and FACS profile.
Serum Free Medium Developed for CD34+ Cell Expansion
StemPro®-34 SFM is comprised of a basal liquid medium and frozen StemPro®-34 Nutrient Supplement is a serum-free media system developed to support the growth of human CD34+ hematopoietic progenitor cells. Sera can vary from lot to lot in its' ability to support cell-specific growth in culture. Serum-free culture systems eliminate this major variable in the study of hematopoiesis ex vivo. StemPro®-34 SFM can be purchased separately.
Included in this kit are:
- One vial containing one ml of 0.5 x10e6 cells/ml pooled donor cord blood-derived CD34+ cells
- 500 ml StemPro®-34 SFM basal liquid medium
- 13 ml frozen StemPro®-34 Nutrient Supplement
For Research Use Only. Not for human or animal therapeutic or diagnostic use.
