|Western blot, immunoprecipitation, immunofluorescence, flow cytometry|
|Human, monkey, mouse, rat|
Host Species and Isotype:
MEK1, also called MAPK or Erk kinase, is a dual-specificity protein kinases that functions in a mitogen activated protein kinase cascade controlling cell growth and differentiation. MEK1 activation occurs through phosphorylation of two serine residues at positions 217 and 221 by Raf-like molecules. MEK1 is phosphorylated by cdk5 at Thr286 in mitotic cells, causing negative feedback of the p44/42 MAP kinase. The MEK1 [pThr286] Antibody detects endogenous levels of MEK1 phosphorylated at threonine 286, and does not cross-react with phosphorylated MEK2. pathway.