|Western blot, immunofluorescence, and immunohistochemistry (paraffin)|
Host Species and Isotype:
p53-binding protein 1 (53BP1) was originally identified as a p53 binding partner that could enhance the transcriptional activity of p53. 53BP1 rapidly translocates to nuclear foci following treatment of cells with ionizing radiation (IR) or radiomimetic agents that cause DNA double strand breaks (DSBs). Recruitment of 53BP1 to sites of DNA damage suggests a role in DSB repair. In response to IR, phosphorylation of 53BP1 at serines 6, 25, 29, and 784 by ATM has been demonstrated, but phosphorylation at these sites is not required for localization of 53BP1 to sites of DSBs. 53BP1 Antibody detects endogenous levels of total 53BP1 protein independent of phosphorylation.