|Western blot, immunoprecipitation, immunofluorescence, flow cytometry|
Host Species and Isotype:
AML1 (also known as Runx1, CBFA2 and PEBP2αB) is a member of the CBF (core binding factor) family of transcription factors (Ref 1,2). It is required for normal development of all hematopoietic lineages (Ref 3,4,5). AML1 forms a heterodimeric DNA binding complex with its partner protein CBFβ and regulates the expression of cellular genes by binding to promoter and enhancer elements. AML1 is commonly translocated in hematopoietic cancers; chromosomal translocations include t(8;21) AML1-ETO, t(12;21)TEL-AML, and t(8;21) AML-M2 (Ref 6). Phosphorylation of AML1 on several potential serine and threonine sites, including Ser249, is thought to occur in an ERK-dependent manner (Ref 7,8).
- Wang, S. et al. (1993) Mol. Cell. Biol .13, 3324-3339.
- Ogawa, E. et al. (1993) Proc. Natl. Acad. Sci. USA 90, 6859-6863.
- Okuda, T. et al. (1996) Cell 84, 321-330.
- Wang, Q. et al. (1996) Proc. Natl. Acad. Sci. USA 93, 3444-3449.
- North, T.E. et al. (2004) Stem Cells 22, 158-168.
- Blyth, K. et al. (2005) Nat. Rev. Cancer 5, 376-387.
- Tanaka, T. et al. (1996) Mol. Cell. Biol. 16, 3967-3979.
- Zhang, Y. et al. (2004) J. Biol. Chem. 279, 53116-53125.