Host Species and Isotype:
c-Kit is a member of the subfamily of receptor tyrosine kinases that includes PDGF, CSF-1, and FLT3/ flk-2 receptors (Ref 1,2). It plays critical controlling roles in a number of cell types such as hematopoietic stem cells, mast cells, melanocytes, and germ cells (Ref 3). Upon binding with its ligand, stem cell factor (SCF), c-Kit undergoes dimerization/ oligomerization, and autophosphorylation. Activation of c-Kit results in the recruitment and tyrosine phosphorylation of downstream SH2-containing signaling components including PLCγ, the p85 subunit of PI3 kinase, SHP2, and CrkL (Ref 4). Molecular lesions that impair the kinase activity of c-Kit are associated with a variety of developmental disorders (Ref 5), while mutations that constitutively activate c-Kit can lead to pathogenesis of mastocytosis and gastrointestinal stromal tumors (Ref 6). Tyr719 is located in the kinase insert region of the catalytic domain. c-Kit phosphorylated at Tyr719 binds to the p85 subunit of PI3 kinase in vitro and in vivo (Ref 7).
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