Phospho-Fak (Tyr 925) rabbit polyclonal antibody recognizes human FAK phosphorylated at tyrosine 925 in western blot, immunoprecipitation, and immunofluorescence experiments.

Antibody Specifications

Applications:

Western blot, immunoprecipitation, immunofluorescence

Species Reactivity:

Human

Host Species and Isotype:

Rabbit IgG

Focal adhesion kinase (FAK) is a widely expressed cytoplasmic protein tyrosine kinase involved in integrin-mediated signal transduction. It plays an important role in the control of several biological processes, including cell spreading, migration, and survival (Ref 1). Activation of FAK by integrin clustering leads to autophosphorylation at Tyr397, which is a binding site for Src family kinases (Ref 2,3), PI3K and PLCγ ( Ref 4,5). The recruitment of Src family kinases results in the phosphorylation of tyrosine residues 407, 576 and 577 in the catalytic domain, and tyrosine residues 871 and 925 in the carboxy terminal region of FAK (Ref 6,7). Phosphorylation of Tyr925 creates a binding site for the Grb2/SH2 domain and triggers a Ras-dependent activation of the MAP kinase pathway (Ref 7).

References:

  1. Parsons, J.T. et al. (2000) Oncogene 19, 5606-5613.
  2. Schaller, M.D. et al. (1994) Mol. Cell. Biol. 14, 1680-1688.
  3. Cobb, B.S. et al. (1994) Mol. Cell. Biol. 14, 147-155.
  4. Chen, H.C. et al. (1996) J. Biol. Chem. 271, 26329-26334.
  5. Zhang, X. et al. (1999) Proc. Natl. Acad. Sci. USA 96, 9021-9026.
  6. Calalb, M.B. et al. (1995) Mol. Cell. Biol. 15, 954-963.
  7. Schlaepfer, D.D. et al. (1994) Nature 372, 786-791.