Sox2 rabbit polyclonal antibody recognizes human and mouse Sox2 in western blot and chromatin immunoprecipitation applications.

Antibody Specifications

Applications:

Western blot, chromatin immunoprecipitation

Species Cross-Reactivity:

Human, mouse

Host Species and Isotype:

Rabbit IgG

Embryonic stem cells are derived from the inner cell mass of the blastocyst, and are unique in their pluripotent capacity and potential for self-renewal. Sox2 is one of a set of genes that is crucial for the maintenance of pluripotency (Ref 1). Sox2, Oct-4 and Nanog co-operate in this network (Ref 1-3), and siRNA knockdown of either Sox2 or Oct-4 results in loss of pluripotency (Ref 4,5). Chromatin immunoprecipitation experiments have shown that Sox2 and Oct-4 bind to thousands of gene regulatory sites, highlighting the importance of these transcription factors in early embryonic development (Ref 6,7).

References:

  1. Nichols, J. et al. (1998) Cell 95, 379-391.
  2. Avilion, A.A. et al. (2003) Genes Dev. 17, 126-140.
  3. Rodda, D.J. et al. (2005) J. Biol. Chem. 280, 24731-24737.
  4. Matin, M.M. et al. (2004) Stem Cells 22, 659-668.
  5. Niwa, H. et al. (2000) Nat. Genet. 24, 372-376.
  6. Boyer, L.A. et al. (2005) Cell 122, 947-956.
  7. Loh, Y.H. et al. (2006) Nat. Genet. 38, 431-440.