Phospho-SHC (Tyr239/240) rabbit polyclonal antibody recognizes human, rat, and mouse phospho-SHC tyrosine 239/240 in western blot.

Antibody Specifications

Applications:

Western blot

Species Cross-Reactivity:

Human, mouse, rat

Host Species and Isotype:

Rabbit IgG

SHC possesses SH2 and PTB domains and serves as a scaffold protein in signaling for a variety of receptor tyrosine kinases. SHC exists in p46, p52, and p66 isoforms, which are produced by using alternative translation initiation sites or a differentially spliced message (Ref 1-3). In response to extracellular signals, the SH2 and PTB domains of SHC interact with the activated receptors, leading to phosphorylation of SHC on three different tyrosine residues: Tyr239, Tyr240, and Tyr317 (Ref 4-6). GRB2/Sos binds to SHC phosphorylated at these sites, activating the Ras/Raf/ MAPK pathway (Ref 4). Both SHC expression and its tyrosine phosphorylation play an essential and nonredundant role in thymic T-cell development (Ref 7).

References:

  1. Pelicci, G. et al. (1992) Cell 70, 93-104.
  2. Migliaccio, E. et al. (1997) EMBO J. 16, 706-716.
  3. Okada, S. et al. (1997) J. Biol. Chem. 272, 28042-28049.
  4. Salcini, A. E. et al. (1994) Oncogene 9, 2827-2836.
  5. Gotoh, N. et al. (1996) EMBO J. 15, 6197-6204.
  6. Van der Geer, P. et al. (1996) Curr. Biol. 6, 1435-1444.
  7. Zhang, L. et al. (2002) Nat. Immunol. 3, 749-755.