FreeStyle™ F17 is an animal origin-free, chemically-defined, protein-free medium specifically developed for the ability to support the growth and transfection of 293-F and CHO cells in suspension without adaptation. This is a multi-platform medium compatible with a range of transient expression systems. The addition of 4-8 mM L-glutamine or GlutaMAX™ supplement is required before use. Gibco® FreeStyle™ F17 Medium features:
• Little or no required adaptation
• Ability to support suspension growth and transfection of 293-F and CHO cells
• Animal origin-free, protein-free and chemically defined formulation
• Scalability in spinner flasks and bioreactors

Little or no required adaptation
Gibco® 293-F, 293-H, FreeStyle™ 293-F and CHO-S cells in suspension can be cultured directly into Gibco® FreeStyle™ F17 Medium without adaptation. Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco® FreeStyle™ F17 Medium, usually without any further adaptation. Cells usually require adaptation from serum-containing formulations.

Ability to support suspension growth and transfection of 293-F and CHO cells
Gibco® FreeStyle™ F17 Medium provides equal or better performance compared to Gibco® FreeStyle™ 293 Expression Medium and Gibco® FreeStyle™ CHO Medium. Gibco® FreeStyle™ F17 Medium supports 293-F cells at a peak density of 6-7 x 10⁶ viable cells per ml, and supports CHO-S cells at a peak density of 8-9 x 10⁶ viable cells per ml. Cells cultured in Gibco® FreeStyle™ F17 can be transfected using a wide variety of transfection reagents, including lipid-based transfection reagents, such as 293fectin™ and Freestyle™ MAX Reagent. Protein expression typically exceeds 75-150 µg/ml IgG in 293-F cells.

Animal origin-free, protein-free and chemically-defined formulation
Gibco® FreeStyle™ F17 Medium is animal origin-free, protein-free and chemically defined, allowing for easier purification of your protein of interest. Gibco® chemically defined media contain no proteins, hydrolysates or components of unknown composition.

Scalability in spinner flasks and bioreactors
Protein production using Gibco® FreeStyle™ F17 Medium can be scaled up in spinner flasks or bioreactors. The appropriate spinner or impeller speed and seeding density should be optimized for each system. Depending on the impeller design and speed, it may be necessary to supplement Gibco® FreeStyle™ F17 Medium with additional Pluronic® F-68 to avoid sheer stress in the culture.

Product Use
For research use/further cell culture manufacturing. CAUTION: Not intended for human or animal diagnostic or therapeutic uses.

cGMP Manufacturing and Quality System
Gibco® FreeStyle™ F17 Medium is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 and ISO 9001 standards.