- Flexible commercial licensing without the need for royalties
- Ability to achieve IgG titers over 3g/L
- Complete workflow that can take your gene of interest from transfection to lead clone typically in 5 months
- Convenient packaging, for simplified ordering and storage
Multiple companies provide CHO (Chinese Hamster Ovary) stable cell line development services and products that require annual fees during development of your therapeutic protein. In addition, royalties may be required on the sales of your therapeutic protein upon commercialization. Gibco® Freedom® Kits provide you with the tools that enable you or your CRO provider to develop your stable cell line for just the price of the kit itself. Furthermore, as you move towards commercial use, a flexible license structure is available without the burden of royalties and works to keep it as simple as a onetime payment (Fig 1). For more information regarding licensing, email us at outlicensing@lifetech.com.
Industry Standard Production Levels
Current bioprocess industry standards to achieve manufacturing efficiencies for IgG type molecule production with stable CHO cell lines in bioreactors is 2-3g/L or better. We have demonstrated that the Gibco® Freedom® CHO-S® Kit can consistently generate IgG producing scalable clones (stable out to 60 population doublings), that meet this standard of productivity (Fig 2).
Complete, Fast, and Efficient Workflow
The Gibco® Freedom® CHO-S® Kit’s complete, proven, and fully integrated workflow allows you to move from transfection to lead clone typically in 5 months, with reduced hands on time, and reduced Fulltime Equivalent (FTE) requirements (Fig 3). The entire workflow is performed in our CD FortiCHO™ medium, a chemically defined, animal origin free (AOF) medium. The kit also offers the following advantages:
- Cloning of one or two genes that encode your protein(s) of interest
- High efficiency transfection of DNA into CHO-S® Cells (cGMP-banked)
- Included flash drive with loaded manual
To free you to focus on the creation of your stable cell line rather than on figuring out where and when all the components of your experiment are arriving, Freedom® CHO-S® Kit introduces the smART™ tote packaging system (shown in above product photos). All components for the creation of your expression cell line are delivered in soft cooler totes along with a parts flyer, and a flash drive containing the manual. Upon delivery you have all the information needed for correct storage and use of components consolidated in the two smART™ tote shipment coolers.
Access to Gibco® Freedom® Kit Support
If any specific support is needed beyond the manual, you can contact your account manager to get a Process Science Fellow consultation. Don’t be surprised if one of our support scientists checks in with you to be sure your experience with the kit is just as successful as possible. For further assistance, please email our support team with your questions and contact information at pd.direct@lifetech.com.
For research use only. CAUTION: Not intended for human or animal diagnostic or therapeutic uses.
