The GeneArt® High-Order Vector Conversion Cassette is a ready-to-use, 3.8 Kb linear BAC⁄YAC cassette, containing yeast replication and selection elements that can make any E.coli
vector compatible with the GeneArt® High-Order Genetic Assembly System
(cat# A13285 & A13286). Creating your compatible vector is as simple as blunt cloning the cassette into the vector’s multiple cloning site. This product only includes the DNA cassette.
Some of the key features and elements of the Conversion Cassette include:
to allow selection of yeast transformants in medium lacking tryptophan
as an optional counter selectable marker in yeast
• ARS⁄CEN origin to allow stable maintenance of the construct in yeast
• Chloramphenicol resistance for selection in E.coli
• 6 restriction enzyme sites, including 2 endonuclease homing sites, designed for rare-cutters to simplify the linearization of the final construct
If you prefer to use a commercial ready-to-use vector, please consider the GeneArt® pYES1L Vector with Sapphire Technology™
(cat# A13287). The vector comes linearized and has been verified to clone up to 110 Kb of DNA fragments.