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Prepare stock solutions with confidence using fresh, single-use size anhydrous DMSO (order D12345)Close
The cell-impermeant, fixable, polar tracer Alexa Fluor® 488 biocytin combines the green-fluorescent Alexa Fluor® 488 fluorophore with biotin and an aldehyde-fixable primary amine. Polar tracers are commonly used to investigate cell-cell and cell-liposome fusion as well as membrane permeability and transport through gap junctions or cell uptake during pinocytosis. This water-soluble tracer can be introduced into cells by whole-cell patch clamping, iontophoresis, osmotic lysis of pinocytic vesicles or comparable methods. The biotin allows for detection and subsequent amplification with enzyme-modified streptavidins including horseradish (HRP) and alkaline phosphatase (AP) that can be used with TSA and ELF technologies, respectively.
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