Lipofectamine® LTX Reagent offers a new, advanced solution for gene expression studies in hard-to-transfect cells. No other plasmid DNA-specific transfection reagent can match the efficiency, convenience, and gentleness of Lipofectamine® LTX Reagent for transfection of primary, difficult-to-transfect, and sensitive cell lines. Lipofectamine® LTX Reagent is synthesized from 100% animal origin-free components, making it easy to validate the absence of zoonotic diseases, such as BSE or viruses, in experiments or cell lines. A certificate of origin may be obtained by contacting Technical Support. Lipofectamine® LTX Reagent offers:
• Superior delivery - transfect a broad range of cell types, including primary and hard-to-transfect cells, with high efficiency
• Superior performance - combine high transfection efficiency with maximal (>90%) cell viability
• Less optimization - rapid, simple protocols provided for most cell lines

Transfect primary cells efficiently
Primary cells have traditionally been difficult to transfect. Lipofectamine® LTX Reagent, when combined with PLUS™ Reagent, solves the problem of transfecting primary cells, including primary neural progenitor cells (Figure 1).

Superior delivery into hard-to-transfect cells
When it comes to achieving high levels of protein expression in RAW267.3 macrophages, Lipofectamine® LTX Reagent outperforms competing transfection reagents (Figure 2). Achieving high-level transgene expression in cells that are traditionally refractory to transfection allows you to obtain results that were previously unattainable.

Broad compatibility with cell lines
In addition to being excellent for transfecting primary and difficult- to-transfect cells, Lipofectamine® LTX Reagent provides you with the most versatile approach to transfecting established cells lines, including those that are sensitive to other transfection reagents (Figure 3). Recommended transfection conditions for these cell types are available at www.invitrogen.com/transfection.

Simple, rapid, serum-compatible protocol
Lipofectamine® LTX Reagent offers a streamlined protocol - no need to remove transfection complexes or change/add medium following transfection. You’ll spend less hands-on time, and get results faster.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.