Compared to trypsin and other dissociation reagents, Gibco® TrypLE™ Select is:
- Gentle on cells—maintains cell health for reproducible results
- Room-temperature stable—convenient to use
- Easy to use—substitutes directly into existing protocols, with no inactivation required
- Animal origin free (AOF)—pure, consistent, and effective
This TrypLE™ Select is modified as follows:
|• EDTA||• Phenol Red|
The formulation of TrypLE™ solutions is available, although we keep the enzyme concentration confidential.
Gentle on cells
TrypLE™ Select, like trypsin, cleaves peptide bonds on the C-terminal sides of lysine and arginine. However, TrypLE™ Select's exceptional purity increases specificity due to the action of a single enzyme. This reduces damage caused by cleavage from multiple enzymes in trypsin and other extracts.
TrypLE™ Select is room-temperature stable and ready to use. TrypLE™ Select cell dissociation reagents remain stable for 24 months at room temperature or 2–8°C, making storage and handling easy and convenient.
Easy to use
TrypLE™ Select can be substituted for trypsin in existing protocols. In addition, dilution alone inactivates TrypLE™ Select, avoiding the need for trypsin inhibitors, such as FBS.
Animal Origin Free (AOF)
Unlike porcine trypsin and other enzymes, TrypLE™ Select is free of animal-derived components, and is formulated on dedicated animal origin-free equipment.
Product Intended Use
For Research Use or Further Manufacturing. Not for use in diagnostic procedures. Customers using Gibco® TrypLE™ Select in a manufacturing process, who have a submission with the FDA, may request a letter of authorization from Life Technologies to reference our Type II Drug Master File (DMF).
cGMP Manufacturing and Quality System
Gibco® TrypLE™ Select is manufactured at a cGMP compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.
Concentrated forms of TrypLE™ can be aseptically diluted to 1x using a balanced salt solution containing 1 mM EDTA.