Blasticidin S is a nucleoside antibiotic isolated from Streptomyces griseochromogenes
. It is a potent inhibitor of protein synthesis in both prokaryotic and eukaryotic cells. Blasticidin S acts quickly by inhibiting peptide bond formation by the ribosome. Blasticidin S is used for the prevention of contamination of cell cultures. The recommended working concentration ranges from 2 to 20 µg / ml depending on the cell line. This product is provided as a powder and should be made into a stock solution at 10 mg/ml in 20mM HEPES (pH 7.2-7.5). Life Technologies offers a variety of antibiotics and antimycotics
for cell culture applications.
In addition, Blasticidin S is used as a bacterial selective antibiotic in the concentration range of 50 – 100 µg / ml. Resistance is conferred by expression of either one of two Blasticidin S deaminase genes: BSD
(2) or bsr
.These deaminases convert Blasticidin S to a nontoxic derivative. For selection purposes, the salt content of the LB medium must remain low (<90 mM) and the pH should not exceed 7.0 to maintain the activity of Basticidin S. Life Technologies also offers a variety of selection antibiotics
. Product Use
For Research Use Only: Not intended for animal or human diagnostic or therapeutic use.