This mouse anti-human Transferrin Receptor monoclonal antibody is specific to human Transferrin Receptor. Transferrin Receptor monoclonal antibody recognizes the expressed product of the TFRC gene. Transferrin is a monomeric serum glycoprotein (MW~80,000 daltons) that binds up to two Fe³⁺ atoms for delivery to vertebrate cells through receptor-mediated endocytosis. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin-receptor complex. Validated applications for Transferrin Receptor monoclonal antibody are immunofluorescence and immunocytochemistry.

Applications: validated for immunofluorescence and immunocytochemistry
Host species and isotype: mouse IgG1.
Clone ID of monoclonal antibody (mAb): 236-15375
Reactivity: human Transferrin Receptor
Product size: 50 µg pack size

Transferrin is a monomeric serum glycoprotein (MW ~80,000 daltons) that binds up to two Fe³⁺ atoms for delivery to vertebrate cells through receptor-mediated endocytosis. Once iron-carrying transferrin proteins are inside endosomes, the acidic environment favors dissociation of iron from the transferrin-receptor complex. Following the release of iron, the apotransferrin is recycled to the plasma membrane, where it is released from its receptor to scavenge more iron. Although fluorescent transferrin conjugates have been widely used to study transferrin receptor-mediated endocytosis, antibodies to the transferrin receptor have proven to be useful for localizing transferrin receptors, studying endocytic recycling pathways and performing enzyme-linked immunosorbent assays (ELISAs). Molecular Probes' anti-human transferrin receptor antibody (mouse monoclonal 236-15375, IgG1) should be useful for the localization of transferrin receptors by immunofluorescence techniques. This antibody, which is purified from ascites by protein A chromatography, recognizes the human transferrin receptor and does not block binding of transferrin to the receptor.