Alexa Fluor® 568 Signal-Amplification Kit for Mouse Antibodies sensitively detects mouse primary antibodies. The kit contains an Alexa Fluor 568 rabbit anti—mouse IgG antibody conjugate, which is used to initially detect a mouse-derived primary antibody. It also contains an Alexa Fluor 568 goat anti—rabbit IgG antibody conjugate, which is used to dramatically amplify the fluorescence signal. Both conjugates have intense orange-red fluorescence with excitation/emission of ~578/603 nm, making them compatible with filters and settings appropriate for Lissamine rhodamine B or Rhodamine Red-X dye. The kit contains a detailed protocol and a sufficient amount of each reagent to stain 150—300 samples of adherent cells grown on coverslips. The kit also contains a protocol for use with flow cytometry.
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