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The BioPrime® Total FFPE Genomic Labeling System is a complete genomic DNA labeling kit designed for use in array comparative genomic hybridization applications (aCGH) using formalin-fixed paraffin-embedded (FFPE) samples. Previous protocols have required large amounts of very high quality DNA, which is often difficult to obtain from FFPE material. With a random prime amplification (RPA) method, using labeling mixes specifically formulated for FFPE samples, BioPrime® Total FFPE Genomic Labeling System generates high yields of labeled DNA which is most representative of the starting sample. Increased call accuracy is achieved with higher yields of labeled material on the array, giving you more accurate calls. The BioPrime® Total FFPE Genomic Labeling System offers users the following advantages: - Improved call rate with higher signal to noise ratios from Alexa Fluor® 3 and 5 dyes - Easy cleanup with PureLink™ purification eliminates the need for complicated volume-reduction steps - Less channel bias due to enhanced reaction formulation for FFPE samples - Lower requirements for input material using enzymatic RPA method This complete, all-inclusive kit is optimized to work across a wider range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component limited reaction allow for consistent, robust DNA yields of genomic FFPE DNA input. BioPrime® Total for Agilent® aCGH is supplied with Purelink™ purification which allows for low elution, eliminating the need for complicated volume-reduction steps. Purelink™ purification also reduces background due to dye bled found in other purification systems An optimized dye labeled nucleotide mix with new dNTP linkers, novel, application-specific Alexa Fluor® 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on arrays. Excitation and emission spectra of Alexa Fluor® 3 and 5 dyes are suited for conventional two color scanners with no need to change settings. Complete kits are batch tested to assure quality in performance from lot to lot.
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