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The BioPrime® Total for Agilent® aCGH system is a complete genomic DNA labeling kit for use in array comparative genomic hybridization applications (aCGH) on the Agilent aCGH platform. Optimized for the Agilent aCGH workflow, arrays, and scanners, BioPrime ® Total for Agilent® aCGH is designed to improve call rates with precious samples. The BioPrime® Total for Agilent® aCGH kit offers users the following advantages:
- Improved call rates from higher signal to noise ratios with Alexa Fluor® 3 and 5 dyes - Less channel bias on Agilent aCGH platform due to novel reaction formulation - Widest range of input material (50ng - 3µg) -Cleanup with PureLink™ purification eliminates the need for complicated volume-reduction steps - Simplified workflow includes master mix formulation and restriction enzymes, Alu I & RSA I
This complete, all-inclusive kit is optimized to work across the widest range of sample input material with no need for pre-amplification. Highly concentrated exo-Klenow fragment of DNA polymerase I in a component limited reaction allows for consistent, robust DNA yields of ~ 8 µg with as little as 50 ng of genomic DNA input. BioPrime® Total for Agilent® aCGH is supplied with Purelink™ purification which allows low elution, eliminating the need for complicated volume-reduction steps. Purelink™ purification also reduces background due to dye bleed-through found in other purification systems. Optimized dye labeled nucleotide mix with new dNTP linkers, novel, application-specific Alexa Fluor® 3 and 5 dyes, and improved buffer chemistry reduce labeling variation and increase signal to noise on Agilent arrays. Excitation and emission spectra of Alexa Fluor® 3 and 5 dyes are suited for Agilent scanners with no need to change settings. Convenient master mix formulation streamlines reaction set-ups increasing consistency. Complete kits include Alu I and RSA I and are batch tested to assure quality in performance from lot to lot.
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