Copy and paste this HTML to embed a link in your website
Paste link in email or IM
Gateway® entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway® entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!
The Gateway® entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway® destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only) • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli • pUC origin for high-copy replication and maintenance of the plasmid in E. coli • Kanamycin resistance gene for selection in E. coli • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for o negative selection and o Chloramphenicol selection in E. coli • Kanamycin resistance gene for selection in E. coli
If you continue without changing your settings, we'll assume that you are happy to receive all cookies on Life Technologies websites. However, you can change your cookie settings at any time at the bottom of this page.
To access this page, please close your session and log into the PunchOut session again. You will need to provide an email address on the sign-in page.Please note if you are a punchout to supply center customer, and are viewing this message, your procurement system does not provide the required information to access this tool. Please contact your procurement system administrator for further instructions.