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One Shot® ccdB Survival™ 2 T1 Phage-Resistant (T1R) chemically competent E. coli are suitable for the propagation of plasmids containing the ccdB gene, and are designed for use with the Gateway® Vector Conversion System and for propagating Gateway® destination, donor, and super-coiled entry vectors. Transformation efficiency is >1 × 109 transformants/ µg DNA. The ccdB Survival™ 2 T1RE. coli strain is derived from the TOP10 strain.
The ccdB Survival™ 2 T1RE. coli strain offers the following Features:
• Resistance to the ccdB gene product • Resistance to T1 and T5 phage (the tonA/fhuA phenotype • Supports high-yield preparation of supercoiled plasmid DNA (the endA1 phenotype) • Reduced non-specific recombination of cloned DNA (the recA1 phenotype)
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