TC-FlAsH™ Expression Analysis Detection Kit - Orange (Fluorescent In-Gel Detection of TC-Tagged and Total Protein)
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TC-FlAsH™ Expression Analysis Detection Kit - Orange is a fluorescent in-gel detection of TC-tagged and total proteins. These detection kits are built on the TC-FlAsH expression tag technology, which utilize a small 6 amino acid TC-Tag and the FlAsH reagent for fast, easy, and specific detection of tagged proteins in gels.
Once host cells are transformed with the TC-tagged expression construct, the crude or purified lysate is incubated with the loading buffer and run on conventional gels.
A UV light box or laser scanner can be used to detect the TC tagged protein at 530 nm and the total protein stain plus benchmark reference ladder at 620 nm.
Sensitivity of the TC-FlAsH is high whether the gel is imaged within the cassette or out of the cassette; UV out of Cassette: 1 ngUV UV within Cassette: 60 ng 488 Laser out of Cassette: 250 pg 488 Laser within Cassette: 4ng
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