This purified recombinant human biotinylated Fab recognizes PRMT3 in human samples.

Recombinant antibody characteristics include:
• Validation in Immunoprecipitation
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Recombinant human biotinylated Fab
• Specific for PRMT3

PRMT3 (protein arginine methyltransferase 3) is one of four type I arginine methyltransferases that catalyse the formation of asymmetric dimethylarginine. PRMTs mediate the Ado Met-dependent methylation of many proteins, including many RNA binding proteins involved in various aspects of RNA processing and/or transport. PRMT3 is unique in that its N-terminus harbours a C2H2 zinc-finger domain that is proposed to confer substrate specificity. In addition, PRMT3 is the only type I enzyme that is restricted to the cytoplasm. The primary structure of each PRMT shares a conserved methyltransferase domain that includes subdomains for binding to the methyl donor, S-adenosyl-l-methionine (SAM), and substrate proteins. Protein arginine methylation has been implicated in signal transduction, nuclear transport, and transcription regulation.

Exceptional Consistency Saves You Time and Money
Recombinant antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.

High Specificity Means More Reliable Data
The recombinant technology employed in manufacture of the antibody assures greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Life Technologies has validated this antibody in immunoprecipitation.

High Sensitivity Lets You Detect Low Levels of Target Protein
Recombinant antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.

Recombinant mouse IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of recombinant antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.

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