This purified recombinant mouse monoclonal antibody recognizes PRMT5 in human samples.

Recombinant antibody characteristics include:
• Validation in immunoprecipitation and mass spectrometry
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Recombinant mouse monoclonal antibody
• Specific for PRMT5

PRMT5 (Protein Arginine Methyltransferase 5) can catalyze the formation of ω-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the former. Targets of symmetrical arginine dimethylation include the small nuclear ribonucleoproteins Sm-D1 and Sm-D3, which require the modification prior to assembly and biogenesis of snRNP core particles. Additional targets of PRMT5-mediated methylation include Piwi proteins (PIWIL1, PIWIL2 and PIWIL4) and histones H2A, H3, and H4. PRMT5 negatively regulates the cyclin E1 promoter.

Exceptional Consistency Saves You Time and Money
Recombinant antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.

High Specificity Means More Reliable Data
The recombinant technology employed in manufacture of the antibody assures greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in immunoprecipitation.

High Sensitivity Lets You Detect Low Levels of Target Protein
Recombinant antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.

Recombinant mouse IgG – Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of recombinant antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

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