Recombinant antibody characteristics include:
• Validation in Immunoprecipitation
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Recombinant mouse monoclonal antibody
• Specific for TRIM28
TRIM28, or Transcription intermediary factor 1-beta, are proteins belonging to the family of tripartite motif (TRIM) super family (1). They are transcriptional regulators that are essential for embryonic development and morphogenesis (2). TRIM28 increases the SUMOylation of IRF7, suggesting that it acts as a SUMO E3 ligase of IRF7, a critical process in the interferon-mediated immune response (3, 4).
Exceptional Consistency Saves You Time and Money
Recombinant antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The recombinant technology employed in manufacture of the antibody assures greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Life Technologies has validated this antibody in immunoprecipitation.
High Sensitivity Lets You Detect Low Levels of Target Protein
Recombinant antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant mouse IgG – Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of recombinant antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
See Life Technologies' full line of ABfinity™ recombinant monoclonal antibodies.
References:
- Herquel B, Ouararhni K, Davidson I. The TIF1α-related TRIM cofactors couple chromatin modifications to transcriptional regulation, signaling and tumor suppression. Transcription. 2011 Sep-Oct;2(5):231-6
- Shibata M, Blauvelt KE, Liem KF Jr, García-García í MJ. TRIM28 is required by the mouse KRAB domain protein ZFP568 to control convergent extension and morphogenesis of extra-embryonic tissues. Development. 2011 Dec;138(24):5333-43
- Liang Q, Deng H, Li X, Wu X, Tang Q, Chang TH, Peng H, Rauscher FJ 3rd, Ozato K, Zhu F. Tripartite motif-containing protein 28 is a small ubiquitin-related modifier E3 ligase and negative regulator of IFN regulatory factor 7. J Immunol. 2011 Nov 1;187(9):4754-63. Epub 2011 Sep 21
- Yaya Chu and Xiaolu Yang. SUMO E3 ligase activity of TRIM proteins. Oncogene. 2011 March 3; 30(9): 1108-1116
