Recombinant antibody characteristics include:
• Validation in Immunoprecipitation
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Recombinant mouse monoclonal antibody
• Specific for FALZ/FALZA
FALZ/FALZA, also known as BPTF or FAC1, is a transcriptional regulator containing a zinc finger motif. It is developmentally regulated (1) and has been shown to be essential for polarity development in mouse trophoblasts (2). Its role in apoptosis was also demonstrated (3). Falz selectively recognizes the methylated/acetylated residues in histones through its C-terminal PHD bromo domain (4).
Exceptional Consistency Saves You Time and Money
Recombinant antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The recombinant technology employed in manufacture of the antibody assures greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Life Technologies has validated this antibody in immunoprecipitation.
High Sensitivity Lets You Detect Low Levels of Target Protein
Recombinant antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant mouse IgG – Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of recombinant antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
See Life Technologies' full line of ABfinity™ recombinant monoclonal antibodies.
References:
- Bowser, R., Giambrone, A., and Davies, P. FAC1, a novel gene identified with the monoclonal antibody Alz50, is developmentally regulated in human brain. Dev Neurosci (1995) 17, 20-37
- Goller T, Vauti F, Ramasamy S, Arnold HH. Transcriptional regulator BPTF/FAC1 is essential for trophoblast differentiation during early mouse development. Mol Cell Biol. 2008 Nov;28(22):6819-27
- Strachan GD, Ostrow LA, Jordan-Sciutto KL. Expression of the fetal Alz-50 clone 1 protein induces apoptotic cell death Biochem Biophys Res Commun. 2005 Oct 21; 336(2):490-5
- Ruthenburg AJ, Li H, Milne TA, Dewell S, McGinty RK, Yuen M, Ueberheide B, Dou Y, Muir TW, Patel DJ, Allis CD. Recognition of a mononucleosomal histone modification pattern by BPTF via multivalent interactions. Cell. 2011 May 27;145(5):692-706
