Recombinant antibody characteristics include:
• Validation in Immunoprecipitation
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Recombinant mouse monoclonal antibody
• Specific for BRD3
BRD3 (Bromodomain-containing protein 3) is expressed ubiquitously and localized in the nucleus. BRD3 recognizes, and binds acetylated lysine residues at the N-terminal tail of histones. The binding of BRD3 results in protein-histone interactions that lead to chromatin remodeling/gene transcription. The brd3 gene is located on chromosome 9. This antibody was developed against a fully folded protein domain, so it does not show good reactivity against denatured proteins, and may not work in western blot applications.
Exceptional Consistency Saves You Time and Money
Recombinant antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The recombinant technology employed in manufacture of the antibody assures greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Life Technologies has validated this antibody in immunoprecipitation.
High Sensitivity Lets You Detect Low Levels of Target Protein
Recombinant antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant mouse IgG – Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of recombinant antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
See Life Technologies' full line of ABfinity™ recombinant monoclonal antibodies.
References:
- The double bromodomain proteins Brd2 and Brd3 couple histone acetylation to transcription.LeRoy G, Rickards B, Flint SJ.,Mol Cell. 2008 Apr 11;30(1):51-60. PMID:18406326
