Recombinant antibody characteristics include:
• Validation in Immunoprecipitation
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Recombinant mouse monoclonal antibody
• Specific for KDM5C
KDM5C, also called as histone demethylase JARID1C, is one among the four JmjN/jmjC proteins that are responsible for the demethylation of H3 histones. The JARID1 family proteins are involved in transcriptional regulation and chromatin remodeling, and JARID1C protein is essential for neural function (1). JARID1C is an X-chromosomal gene and it is homologous to the Y-chromosomal gene JARID1D. This protein contains several conserved domains including a JmjN domain, an Arid/Bright domain, a C5HC2 zinc-finger domain, and PHD zinc-finger domains (2).
Exceptional Consistency Saves You Time and Money
Recombinant antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The recombinant technology employed in manufacture of the antibody assures greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Life Technologies has validated this antibody in immunoprecipitation.
High Sensitivity Lets You Detect Low Levels of Target Protein
Recombinant antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant mouse IgG – Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of recombinant antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
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References:
- Secombe J, Eisenman R. The function and regulation of the JARID1 family of Histone H3 Lysine 4 Demethylases. Cell Cycle 2007 June 6(11): 1324-1328.
- Jensen R, et al. Mutations in the JARID1C Gene, Which Is Involved in Transcriptional Regulation and Chromatin Remodeling, Cause X-Linked Mental Retardation Am. J. Hum. Genet. Q J Med 2005 76: 227-236.
