Recombinant antibody characteristics include:
• Validation in Immunoprecipitation
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Recombinant mouse monoclonal antibody
• Specific for CBX3
CBX-3, or Chromobox homolog 3, is encoded by the cbx3 gene located on chromosome 7. CBX 3 is also known as Heterochromatin protein 1 homolog gamma (HP1). This protein is ubiquitously expressed and localized in the nucleus (1). CBX-3 is an important component of heterochromatin and due to its dual binding capacity can bind heterochromatin to the lamin B receptor present on the inner nuclear membrane. This protein also mediates chromatin remodeling and DNA dependent negative regulation of transcription by binding to methylated lys-9 residues in histone tails (2). CBX 3 is also known to play a role in DNA damage repair induced by UV radiation or double strand breaks.
Exceptional Consistency Saves You Time and Money
Recombinant antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The recombinant technology employed in manufacture of the antibody assures greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Life Technologies has validated this antibody in immunoprecipitation.
High Sensitivity Lets You Detect Low Levels of Target Protein
Recombinant antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant mouse IgG – Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of recombinant antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
See Life Technologies' full line of ABfinity™ recombinant monoclonal antibodies.
References:
- Methylation of histone H3 lysine 9 creates a binding site for HP1 proteins. Lachner M., O'Carroll D., Rea S., Mechtler K., Jenuwein T. Nature 410, 116-120 (2001) [PubMed:11242053]
- Localization and phosphorylation of HP1 proteins during the cell cycle in mammalian cells.Minc E., Allory Y., Worman H.J., Courvalin J.C., Buendia B. Chromosoma 108, 220-234 (1999) [PubMed:10460410]
