This purified, unconjugated rabbit anti-connexin 30 polyclonal antibody is specific to human, mouse, rat and feline connexin 30. Cx30 is highly expressed in adult skin and brain. Anti-connexin 30 polyclonal antibody recognizes the expressed product of the GJB6 gene. Validated applications for rabbit anti-connexin 30 polyclonal antibody are western blotting, ELISA, and immunohistochemistry (frozen).
• Applications: Validated applications for anti-connexin 30 polyclonal antibody are western blotting, ELISA, and immunohistochemistry (frozen).
• Host Species and Isotype: The host species and isotype of the anti-connexin 30 polyclonal antibody is rabbit, IgG.
• Also Known As: Connexin 30 is also known as ED2, EDH, HED, CX30, DFNA3, DFNA3B, and DFNB1B.
• Reactivity: Reacts with human, feline, mouse, and rat connexin-30
• Product Size: Rabbit anti-connexin 30 polyclonal antibody is available in a 50 µg size.
Intracellular communication mediated by gap junctions plays an important role in a variety of cellular processes including: homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are trans-membrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemi-channels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family composed of at least 12 distinct members. Each of the connexins exhibits similar structural features including a cytoplasmic amino terminal region, four trans-membrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. Comparison of the amino acid sequences of the various connexin family members indicate that the two areas of greatest divergence amongst the connexin family members are the intracellular loop connecting the second and third trans-membrane segments and the carboxy-terminal tail. These domains are, therefore, thought to mediate connexin-type specific properties including: phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of Gap junctional communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, postranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has be found to correlate with tumor progression and metastasis.
Connexin 30 (Cx-30) is a newly identified member of the connexin gene family. Cx-30 was isolated by screening a genomic mouse library with a rat Cx26 probe. The Cx30 protein is most closely related to the Cx26 protein (77% amino acid sequence identity). The expression patterns of Cx30 and Cx26 are clearly distinct, specifically, Cx30 is highly expressed in adult skin and brain; however, it cannot be detected in the embryonic and fetal brain. On the other hand, Cx26 is expressed highly in prenatal brain and decreases after birth.