This purified Rabbit anti-ZO-2 Polyclonal Antibody is specific to human, canine, and mouse ZO-2. Studies have shown that canine ZO-2 is 87% identical to the protein encoded by the human gene X104 related to the Friedreich ataxia locus; therefore, the X104 gene product is thought to be the human homolog of canine ZO-2. Validated applications of this rabbit anti-ZO-2 polyclonal antibody are ELISA, Western blotting and immunohistochemistry.

Antibody Attributes:

• Applications: Validated applications of this rabbit anti-ZO-2 polyclonal antibody are ELISA, Western blotting and immunohistochemistry.
• Host Species and Isotype: The host species and isotype of the ZO-2 polyclonal antibody is rabbit IgG.
• PAD: The ZO-2 Polyclonal Antibody PAD is Z54.PL.
• Reactivity: This rabbit anti-ZO-2 polyclonal antibody is specific to human, canine, and mouse ZO-2.
• Product Size: ZO-2 polyclonal antibody, rabbit is available in a 100 µg pack size.

Tight junctions play dual roles in the physiological functions of both epithelial and endothelial cells by creating a barrier to the diffusion of solutes through the paracellular pathway, and by functioning as a boundary between the apical and basolateral plasma membrane. The first TJ protein to be identified was the 220 kDa peripheral membrane protein ZO-1, localized at TJs in both epithelial and endothelial cells. This protein is also expressed in cells which lack TJs such as fibroblasts; however, in these cell types, the ZO-1 protein is localized at adherens junctions. Later studies revealed the existence of a ZO-1 homologue known as ZO-2. ZO-2 is also a peripheral membrane protein; however, unlike ZO-1, ZO-2 is found only at TJ. In addition to ZO-1 and ZO-2, other TJ-specific proteins are occludin, cingulin, the 7H6 antigen, Rab3B and symplekin.

The ZO-1 and ZO-2 proteins share significant sequence homology to each other (51% identity), as well as to several other proteins which include the lethal(1)discs-large(dlg) tumor suppressor gene product of Drosophila, erythrocyte membrane-associated p55 protein, and PSSD-95/SAP90.14-17 These proteins share several conserved domains and are collectively referred to as the MAGUK (membrane associated guanylate kinase-containing) family. The regions of homology include a domain homologous to guanylate kinase (GUK), a single SH3 domain, and a variable number of N-terminal repeated PDZ domains. Although ZO-1 and ZO-2 are structurally similar, the proteins are divergent at their C-termini, suggesting that the C-terminal ends of ZO-1 and ZO-2 may have distinct functions.

The canine ZO-2 protein has a predicted molecular mass of ~132 kDa; however, the protein migrates with a mass of 160kDa when analyzed by SDS-PAGE. Studies have shown that canine ZO-2 is 87% identical to the protein encoded by the human gene X104 related to the Friedreich ataxia locus; therefore, the X104 gene product is thought to be the human homolog of canine ZO-2.