This purified rabbit anti-connexin-43 polyclonal antibody is specific to rat, mouse, and human connexin 43. Anti-connexin 43 polyclonal antibody recognizes the product of the GJA1 gene. Reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis. This connexin-43 polyclonal antibody is validated for use in western blotting, immunohistochemistry, immunoassay (ELISA), immunocytochemistry, and immunofluorescence.
• Applications: Validated applications for connexin-43 polyclonal antibody are western blotting, immunohistochemistry, immunoassay (ELISA), and immunocytochemistry/immunofluorescence.
• Host Species: The host species of this connexin-43 polyclonal antibody is rabbit.
• Purification: This polyclonal antibody is affinity purified.
• Reactivity: Detects human, mouse, and rat connexin-43.
• Product Size: Rabbit anti-connexin-43 polyclonal antibody is available in a 50 µg pack size.
Intracellular communication mediated by gap junctions plays an important role in a variety of cellular processes including homeostasis, morphogenesis, cell differentiation, and growth control. Gap junctions are transmembrane channels that serve to directly link neighboring cells by mediating the exchange of low-molecular weight metabolites, ions, and second messengers. Gap junctions are formed by the interaction of connexons or hemichannels on adjacent cells. The connexon itself is composed of a hexameric assembly of proteins referred to as connexins. Connexins are highly homologous proteins encoded by a multigene family. The connexins exhibit similar structural features which include a cytoplasmic amino terminal region, four transmembrane domains, two extracellular loops, and a carboxy-terminal cytoplasmic tail of varying length. Comparison of the amino acid sequences of the various connexin family members indicate that the two areas of greatest divergence amongst the connexin family members are the intracellular loop connecting the second and third transmembrane segments and the carboxy-terminal tail. These domains are thought to mediate connexin-type specific properties including phosphorylation, responses to gating stimuli, as well as assembly and membrane turnover. Modulation of gap junctional communication can be achieved by multiple mechanisms and can occur very rapidly or over a period of several hours. These mechanisms include alterations in transcription, translation, stability, postranslational processing (especially phosphorylation), gating, and insertion or removal from the plasma membrane. Interestingly, reduction or alterations in the levels or types of connexin expressed in a given cell type has been found to correlate with tumor progression and metastasis.