This purified oligoclonal recombinant rabbit IgG antibody recognizes human EIF2a [pS51]

Antibody Specifications:

• Validated in Immunocytochemistry, Western Blot and indirect ELISA
• Isotype: Rabbit IgG
• Concentration: 0.5 mg/ml
• Buffer: Phosphate-buffered saline (PBS) with 0.09% sodium azide

Oligoclonal Antibodies—Exceptional Consistency Helps Save Time and Money
Invitrogen's oligoclonal antibodies are a pool of monoclonal rabbit IgG antibodies targeted against a specific antigen. Oligoclonal antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.

Oligoclonal Antibody Advantages Include:

• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Validation by Invitrogen

Specific for EIF2a [pS51]
EIF2A is a heterotrimer composed of three subunits (alpha, beta, and gamma) encoding the eukaryotic initiation factor 2A. This translation initiation factor was purified from rabbit reticulocytes and shown to drive binding of initiator methionyl-tRNA (met-tRNAi) to the 40S ribosome in an AUG-dependent manner to form 43S pre-initiation complex (1). This polypeptide can be phosphorylated by related protein kinases activated in response to stress. Phosphorylated EIF2A inhibits EIF2B activity and prevents guanine nucleotide exchange (2).

Obtain More Reliable Data
The proprietary recombinant technology employed in the production of Invitrogen’s oligoclonal antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved.

Detect Low Levels of Target Protein
Invitrogen's oligoclonal antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.

Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of rabbit oligoclonal antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

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References:
Merrick WC, Anderson WF (1975) Purification and characterization of homogeneous protein synthesis initiation factor M1 from rabbit reticulocytes. J Biol Chem 250: 1197–1206.
Wek RC et al., (2006)Coping with stress: eIF2 kinases and translational control. Biochem Soc Trans. 2006 Feb;34(Pt 1):7-11