His Tag Oligoclonal Antibody, ABfinity™ Recombinant
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The purified rabbit anti-His Tag Oligoclonal Antibody ABfinity™ Recombinant recognizes His tag. His tag is a synthetic oligo peptide consisting of 6 consecutive histidine residues (HHHHHH). It is commonly expressed as a tag at either N-or C- terminus.This rabbit anti-His Tag Oligoclonal Antibody ABfinity™ Recombinant can be used in applications such as Western blotting and indirect ELISA.
Applications: Validated applications for rabbit anti-His Tag Oligoclonal Antibody ABfinity™ Recombinant are Western blotting and indirect ELISA. Host species and isotype: The host species of the rabbit anti-His Tag Oligoclonal Antibody ABfinity™ Recombinant is rabbit and isotype is IgG. Reactivity: Rabbit anti-His Tag Oligoclonal Antibody ABfinity™ Recombinant detects His tag. Product size: Rabbit anti-His Tag Oligoclonal Antibody ABfinity™ Recombinant is available in a 100 µg pack size.
Concentration: 0.5 mg/ml Buffer: Phosphate-buffered saline (PBS) with 0.09% sodium azide Oligoclonal Antibodies—Exceptional Consistency Helps Save Time and Money
Life Technologies' oligoclonal antibodies are a pool of monoclonal rabbit IgG antibodies targeted against a specific antigen. Oligoclonal antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
Oligoclonal Antibody Advantages Include:
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Validation by Life Technologies Specific for His Tag
His tag is a synthetic oligo peptide which has 6 consecutive Histidine residues (HHHHHH). It can be expressed as part of a recombinant protein at either the N- or C-terminus, depending on the vector. His tag fusion is often used for isolation, detection and/or purification of proteins by the IMAC method. Poly-histidine has an affinity for Ni2+/Co2+/Cu2+ ions, and (His)6-tagged fusion proteins bind to metal-charged IMAC resin.
Obtain More Reliable Data
The proprietary recombinant technology employed in the production of Life Technologies' oligoclonal antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved.
Detect Low Levels of Target Protein
Life Technologies' oligoclonal antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of rabbit oligoclonal antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
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