- Applications: Western blot and indirect ELISA
- Host Species and Isotype: Rabbit IgG
- Species Cross-reactivity: Human
Life Technologies' oligoclonal antibodies are a pool of monoclonal rabbit IgG antibodies targeted against a specific antigen. Oligoclonal antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
Oligoclonal Antibody Advantages Include:
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
Mammalian target of Rapamycin (mTOR), also known as FKBP12-rapamycin-associated protein (FRAP), is a ~280 kDa serine/threonine kinase and a key modulator of cell growth and protein synthesis. mTOR is implicated in cancer and neurite plasticity and plays a central role in mediating phosphoinositide 3-kinase (PI3 kinase) and Akt/PKB signals for cell growth, proliferation and protein translation via ribosomal S6 kinase (S6Ks) and translation regulator eIF4E-binding protein 1. mTOR is differentially phosphorylated on threonine 2446 and serine 2448 in response to nutrient status and growth factor stimulation. Serine 2448 is located in the catalytic domain and is phosphorylated by Akt/PKB and in response to insulin, glucagons and amino acid stimulation.
Obtain More Reliable Data
The proprietary recombinant technology employed in the production of Life Technologies' oligoclonal antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved.
Detect Low Levels of Target Protein
Life Technologies' oligoclonal antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG-Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of rabbit oligoclonal antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
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