- Applications: Western blot and indirect ELISA
- Host Species and Isotype: Rabbit IgG
- Species Cross-reactivity: Human
Life Technologies' oligoclonal antibodies are a pool of monoclonal rabbit IgG antibodies targeted against a specific antigen. Oligoclonal antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
Oligoclonal Antibody Advantages Include:
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
Epidermal Growth Factor Receptor (EGFR) is 175 kDa transmembrane glycoprotein and belongs to tyrosine kinase superfamily. EGFR acts as a receptor for epidermal growth factor (EGF) family. Binding of EGFR to its ligands causes autophosphorylation of tyrosine kinase followed by activation of signal transduction of the pathways connected to cell proliferation and differentiation. Phosphorylation at Tyrosine 1148, activated EGFR and increases tyrosine kinase activity towards a variety of intracellular substrates. Deregulation of EGFR expression has been connected to carcinogenesis and metastasis. EGFR has been a major focus for targeted cancer therapy in recent times.
Obtain More Reliable Data
The proprietary recombinant technology employed in the production of Life Technologies' oligoclonal antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved.
Detect Low Levels of Target Protein
Life Technologies' oligoclonal antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG-Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of rabbit oligoclonal antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
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