- Applications: Western blot, Indirect ELISA
- Host Species and Isotype: Rabbit IgG
- Species Cross-reactivity: Human
Life Technologies' oligoclonal antibodies are a pool of monoclonal rabbit IgG antibodies targeted against a specific antigen. Oligoclonal antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
Oligoclonal Antibody Advantages Include:
- Validation in Western blot
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Recombinant rabbit monoclonal antibody
Protein p21 is a tumor suppressor protein which is an inhibitor of Cyclin-dependent kinases (CDK) and is transcriptionally activated by p53. It is important in the response of cells to genotoxic stress and a major transcriptional target of p53 protein. The occurrence of p21 in nucleus executes binding and inhibiting the activity of cyclin dependent kinases Cdk1 and Cdk2, and blocks the transition from G1 phase into S phase or from G2 phase into mitosis after DNA damage. This enables the repair of damaged DNA. In the cytoplasm, p21 protein has an anti-apoptotic effect. It is able to bind to and inhibit caspase 3, as well as the apoptotic kinases ASK1 and JNK. P21 also exhibits a dual function in cancerogenesis. It acts as a tumor suppressor, prevents apoptosis, and acts as an oncogene.
Obtain More Reliable Data
The proprietary recombinant technology employed in the production of Life Technologies' oligoclonal antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved.
Detect Low Levels of Target Protein
Life Technologies' oligoclonal antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG-Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of rabbit oligoclonal antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
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