Antibody Specifications:
- Applications: Western blot and indirect ELISA
- Host Species and Isotype: Rabbit IgG
- Species Cross-reactivity: Human
Life Technologies' oligoclonal antibodies are a pool of monoclonal rabbit IgG antibodies targeted against a specific antigen. Oligoclonal antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
Oligoclonal Antibody Advantages Include:
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
Pax 3 (Paired Box 3) is a transcription factor that is required by pre-migratory neural crest cells that give rise to the peripheral nervous system, melanocytes, some vascular smooth muscle, and numerous other derivatives, and is localized in the dorsal neural tube and in myogenic progenitors in the presomitic mesoderm and the hypaxial somites. Repressors of Pax 3 transcriptional activity include the transcriptional co-repressor hDaxx, the retinoblastoma protein (Rb) and Calmyrin. The Pax3-FKHR fusion protein is formed due to chromosomal translocation and induces morphological change and enhances cellular proliferation and invasion in alveolar rhabdomyosarcoma (ARMS) development. Pax 3 mutations causes Waardenburg Syndrome, which is an auditory-pigmentary syndrome caused by a deficiency of melanocytes and other neural crest-derived cells. Pax 3 is also involved in the development and progression of malignant melanoma.
Obtain More Reliable Data
The proprietary recombinant technology employed in the production of Life Technologies' oligoclonal antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved.
Detect Low Levels of Target Protein
Life Technologies' oligoclonal antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG-Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of rabbit oligoclonal antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
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