Antibody Specifications:
- Validated in Western Blot and Indirect ELISA
- Isotype: Rabbit IgG
- Concentration: 0.5 mg/ml
- Buffer: Phosphate-buffered saline (PBS) with 0.09% sodium azide
Oligoclonal Antibodies—Exceptional Consistency Helps Save Time and Money
Invitrogen's oligoclonal antibodies are a pool of monoclonal rabbit IgG antibodies targeted against a specific antigen. Oligoclonal antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
Oligoclonal Antibody Advantages Include:
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Validation by Invitrogen
Specific for PRAS40 [pT246]
Proline Rich Akt Substrate of 40 kDa (PRAS40) is a signaling target known to play various regulatory roles in growth, development, and apoptosis by inducing phosphoinositol kinase 3 pathway (Ref 1,2). PRAS40 also acts by binding to mTOR complex 1 and inhibiting mTOR function by altering nutrient balance and growth maintenance (Ref 3). Phosphorylation of PRAS40 by mTORC1 at Serine 183, 212, and 221 residues disrupts this binding thus removing the inhibitory effect. Activated Akt phosphorylates PRAS40 on threonine 246. Mutation of PRAS40 threonine 246 has been shown to be apoptotic, and plays a role in abrogating neuronal protection (Ref 4).
Obtain More Reliable Data
The proprietary recombinant technology employed in the production of Invitrogen’s oligoclonal antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved
Detect Low Levels of Target Protein
Invitrogen's oligoclonal antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of rabbit oligoclonal antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Related Link:
Rapidly Locate the Antibody You Need Using the Antibody Search Tool
References:
- Dunlop EA, Tee AR. Mammalian target of rapamycin complex 1: signalling inputs, substrates and feedback mechanisms. Cell Signal. 2009 Jun;21(6):827-35. Epub 2009 Jan 8. Review. PubMed PMID: 19166929.
- New DC, Wu K, Kwok AW, Wong YH. G protein-coupled receptor-induced Akt activity in cellular proliferation and apoptosis. FEBS J. 2007 Dec;274(23):6025-36. Review. PubMed PMID: 17949438.
- Wang L, Harris TE and Lawrence JC Jr. Identification of two sites of PRAS40 phosphorylated by mTOR complex 1. The FASEB Journal. 2008; 22: 911.6.
- Kovacina KS, Park GY, Bae SS, Guzzetta AW, Schaefer E, Birnbaum MJ, Roth RA. Identification of a proline-rich Akt substrate as a 14-3-3 binding partner. J Biol Chem. 2003 Mar 21;278(12):10189-94. PubMed PMID: 12524439.
