Antibody Specifications:
- Validated in Western Blot
- Isotype:
Invitrogen’s oligoclonal antibodies are a pool of monoclonal rabbit IgG antibodies targeted against a specific antigen. Oligoclonal antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
Oligoclonal Antibody Advantages Include:
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Validation by Invitrogen
MDM2, also known as "murine double minute 2," interacts with and negatively regulates the function of the tumor suppressor protein p53 (1). MDM2 binds to p53 thereby inhibiting transactivation; overexpression of MDM2 can result in the inactivation of p53, decreasing tumor suppression. MDM2 also inactivates p53 through degradation, as MDM2 possesses E3 ubiquitin ligase activity. On interacting with other proteins, MDM2 has shown effects upon apoptosis and the cell cycle (2). The gene encoding MDM2 is located on the q arm of chromosome 12 (3).
Obtain More Reliable Data
The proprietary recombinant technology employed in the production of Invitrogen’s oligoclonal antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved
Detect Low Levels of Target Protein
Invitrogen’s oligoclonal antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG--Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of rabbit oligoclonal antibodies produces a 150kDa band under non-reducing conditions and 50kDa and 25kDa bands under reducing conditions.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Related Link:
Rapidly Locate the Antibody You Need Using the Antibody Search Tool
References:
- Manfredi JJ. The Mdm2-p53 relationship evolves: Mdm2 swings both ways as an oncogene and a tumor suppressor. Genes Dev. 2010 Aug 1;24(15):1580-9. Review. Erratum in: Genes Dev. 2010 Sep 15;24(18):2105. PubMed PMID: 20679392
- Lozano G, Montes de Oca Luna R. MDM2 function. Biochim Biophys Acta. 1998 Apr 17;1377(2):M55-9. Review. PubMed PMID: 9606977
- Weaver J, Downs-Kelly E, Goldblum JR, Turner S, Kulkarni S, Tubbs RR, Rubin BP, Skacel M. Fluorescence in situ hybridization for MDM2 gene amplification as a diagnostic tool in lipomatous neoplasms. Mod Pathol. 2008 Aug;21(8):943-9. Epub 2008 May 23. PubMed PMID: 18500263
