This purified ABfinity™ recombinant rabbit monoclonal antibody recognizes MAP2K1 [pSpS217/221] in human samples.

ABfinity™ antibody characteristics include:

• Validation in Western blot and indirect ELISA
• Superior lot-to-lot consistency
• Extremely high specificity and sensitivity
• Recombinant rabbit monoclonal antibody
• Specific for MAP2K1 [pSpS217/221]

MAP2K1 [pSpS217/221] is a dual specificity mitogen-activated protein kinase (MAPK) kinase also known as MEK. It is an essential component of the MAP kinase pathway and stimulates the enzymatic activity of MAP kinases in response to signals. Growth factors, cytokines, and proto-oncogenes transduce their signals through the activation of RAS, which leads to activation of the serine/threonine kinase RAF, and then to activation of MAPK kinase or MEK. This kinase is activated by Raf family member p74raf-1 through phosphorylation of Ser residues at positions 217 and 221 in the activation loop. MEK phosphorylates and activates MAPK, also known as extracellular signal-regulated kinase (ERK) (1).

Exceptional Consistency Saves You Time and Money
ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.

High Specificity Means More Reliable Data
The proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting.

High Sensitivity Lets You Detect Low Levels of Target Protein
ABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.

Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.

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References:
Alessi DR et al., EMBO J. 1994 Apr 1;13(7):1610-9.Identification of the sites in MAP kinase kinase-1 phosphorylated by p74raf-1.