ABfinity™ antibody characteristics include:
- Validation in Western blot and indirect ELISA
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Recombinant rabbit monoclonal antibody
Insulin receptor (IR, CD220) belongs to the superfamily of growth factor receptor tyrosine kinases, which regulates multiple signaling pathways (Ref 1). It is a heterotetrameric membrane glycoprotein consisting of α and β subunits, which are encoded by the single gene INSR in humans. IR and IGF-1 receptor share major structural and functional similarity (Ref 2). The catalytic loops within the tyrosine kinase domains of the IR/IGF-1R share the same residue sequence with a three-tyrosine motif corresponding to Tyr1158, 1162, and 1163 (for IR) and Tyr1131, 1135, and 1136 (for IGF-1R). It is generally believed that autophosphorylation within the activation loop proceeds in a progressive manner: initiation at the second tyrosine (1162 or 1135), followed by phosphorylation at the first tyrosine (1158 or 1131), then the last (1163 or 1136), whereupon the IR or IGF-1R becomes fully active (Ref 3). In humans, the IR gene is present on chromosome 19 (Ref 4).
Exceptional Consistency Saves You Time and Money
ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting.
High Sensitivity Lets You Detect Low Levels of Target Protein
ABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies
See Invitrogen's full line of ABfinity™ recombinant monoclonal antibodies
References:
- Giudice J, Leskow FC, Arndt-Jovin DJ, Jovin TM, Jares-Erijman EA. Differential endocytosis and signaling dynamics of insulin receptor variants IR-A and IR-B. J Cell Sci. 2011 Mar 1;124(Pt 5):801-11. PubMed PMID: 21303927
- Moxham CP, Duronio V, Jacobs S. Insulin-like growth factor I receptor beta-subunit heterogeneity. Evidence for hybrid tetramers composed of insulin-like growth factor I and insulin receptor heterodimers. J Biol Chem. 1989 Aug 5;264(22):13238-44. PubMed PMID: 2546949
- Morrison BD, Pessin JE. Insulin stimulation of the insulin receptor kinase can occur in the complete absence of beta subunit autophosphorylation. J Biol Chem. 1987 Feb 25;262(6):2861-8. PubMed PMID: 3546299
- Straus DS, Pang KJ, Kull FC Jr, Jacobs S, Mohandas T. Human insulin receptor gene. Data supporting assignment to chromosome 19. Diabetes. 1985 Aug;34(8):816-20. PubMed PMID: 4018417
