ABfinity™ antibody characteristics include:
- Validation in Western Blot and indirect ELISA
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Recombinant rabbit monoclonal antibody
GSK3b belongs to the subfamily of glycogen synthase kinases, which plays a central role in energy metabolism, neuronal cell development, and body pattern formation (Ref 1). GSK3a and GSK3b are two isoforms of sizes 51 kDa and 47 kDa respectively, but they are not functionally identical (Ref 2). GSK3 beta plays a key role in insulin and Wnt signaling; it phosphorylates downstream targets by default, and becomes inhibited following extracellular signaling events. Phosphorylation at the S9 position of GSK3b has been shown to inhibit its kinase activity (Ref 3).
Exceptional Consistency Saves You Time and Money
ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting.
High Sensitivity Lets You Detect Low Levels of Target Protein
ABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
See Invitrogen's full line of ABfinity™ recombinant monoclonal antibodies.
References:
- Billadeau DD. Primers on molecular pathways. The glycogen synthase kinase 3beta. Pancreatology. 2007;7(5-6):398-402. Review. PubMed PMID: 17912008.
- Doble BW, Woodgett JR. GSK-3: tricks of the trade for a multi-tasking kinase. J Cell Sci. 2003 Apr 1;116(Pt 7):1175-86. Review. PubMed PMID: 12615961.
- Kim L, Kimmel AR. GSK3 at the edge: regulation of developmental specification and cell polarization. Curr Drug Targets. 2006 Nov;7(11):1411-9. Review. PubMed PMID: 17100581.
