ABfinity™ antibody characteristics include:
- Validation in Western blot and Indirect ELISA
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Recombinant rabbit monoclonal antibody
p38 Mitogen-activated protein kinases, also known as SAPK2 (Stress-Activated Protein Kinase 2), belongs to the mitogen-activated protein kinase family, which is responsive to stress stimuli. The mammalian p38 MAPK families are activated by cellular stress including UV irradiation, heat shock, high osmotic stress, lipopolysaccharide, protein synthesis inhibitors, proinflammatory cytokines (such as IL-1 and TNF-alpha), and certain mitogens, which may lead to autoimmunity. There are four p38 kinases - α, β, γ and δ. p38α is well characterized and is expressed in most cell types. The p38 MAPK subfamily plays important roles in cytokine production and the stress response. Additional functions for p38 MAPKs are in the inhibition of cell cycle progression, developmental processes, and the differentiation of several vertebrate cell types. An important target of the p38 MAP kinase is the tumor suppressor protein p53, whose activation is positively regulated by p38. MKK3, MKK6, and SEK phosphorylate p38 MAPK at threonine 180 and tyrosine 182. Phosphorylation of these residues activates p38 MAPK which in turn activates and phosphorylates ATF1, ATF2, and ATF6.
Exceptional Consistency Saves You Time and Money
ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting.
High Sensitivity Lets You Detect Low Levels of Target Protein
ABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG-Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
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