ABfinity™ antibody characteristics include:
- Validation in Western blot and Indirect ELISA
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Recombinant rabbit monoclonal antibody
Protein disulfide isomerase (PDI), an enzyme present in the endoplasmic reticulum (ER), plays an important role in protein folding by facilitating formation of correct disulphide bonds (Ref 1,3). PDI catalyses thiol/disulphide interchange interactions (Ref 3). Depending upon the type of polypeptide substrate and redox potential, it will cause protein disulfide formation, isomerization, or reduction (Ref 3). The PDI family of enzymes, which share homology with the cytoplasmic protein thioredoxin, actively catalyze the processing and maturation of secretory proteins in the endoplasmic reticulum (Ref 2).
Exceptional Consistency Saves You Time and Money
ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting.
High Sensitivity Lets You Detect Low Levels of Target Protein
ABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
See Invitrogen's full line of ABfinity™ recombinant monoclonal antibodies.
References:
- Wang Q, Chang A. Eps1, a novel PDI-related protein involved in ER quality control in yeast. EMBO J. 1999 Nov 1;18(21):5972-82. PubMed PMID: 10545109.
- Ferrari DM, Söling HD. The protein disulphide-isomerase family: unravelling a string of folds. Biochem J. 1999 Apr 1;339 ( Pt 1):1-10. Review. PubMed PMID.
- Gething MJ, Sambrook J. Protein folding in the cell. Nature. 1992 Jan 2;355 (6355):33-45. Review. PubMed PMID: 1731198.
