ABfinity™ antibody characteristics include:
- Validation in Western blot
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Recombinant rabbit monoclonal antibody
Osteonectin is a matrix-associated protein also known as Secreted Protein Acidic and Rich in Cysteine (SPARC), and Basement Membrane tumor matrix component (BM-40). Though this noncollagenous glycoprotein plays a very important role in bone mineralization, it is also expressed in other tissues performing other functions such as tissue repair, remodeling of tissue, and development (1,2). It is a multifunctional protein with a high affinity for cations and hydroxyapatite; it provides support to extracellular matrix and mediates the activities of a wide range of growth factors (3). In addition, osteonectin plays a vital role in tumor formation (4).
Exceptional Consistency Saves You Time and Money
ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting.
High Sensitivity Lets You Detect Low Levels of Target Protein
ABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150kDa band under non-reducing conditions and 50kDa and 25kDa bands under reducing conditions.
For research use only. Not intended for any human or animal therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ Recombinant Monoclonal Antibodies
See Invitrogen’s full line of ABfinity™ Recombinant Monoclonal Antibodies
References:
- Yan Q, Sage EH. SPARC, a matricellular glycoprotein with important biological functions. J Histochem Cytochem. 1999 Dec;47(12):1495-506. Review. PubMed PMID: 10567433.
- Villarreal XC, Mann KG, Long GL. Structure of human osteonectin based upon analysis of cDNA and genomic sequences. Biochemistry. 1989 Jul 25;28(15):6483-91. PubMed PMID: 2790009.
- Bradshaw AD, Sage EH. SPARC, a matricellular protein that functions in cellular differentiation and tissue response to injury. J Clin Invest. 2001 May;107(9):1049-54. Review. PubMed PMID: 11342565; PubMed Central PMCID: PMC209289.
- Gilmour DT, Lyon GJ, Carlton MB, Sanes JR, Cunningham JM, Anderson JR, Hogan BL, Evans MJ, Colledge WH. Mice deficient for the secreted glycoprotein SPARC/osteonectin/BM40 develop normally but show severe age-onset cataract formation and disruption of the lens. EMBO J. 1998 Apr 1;17(7):1860-70. PubMed PMID: 9524110.
