ABfinity™ antibody characteristics include:
- Validation in Western blot and Indirect ELISA
- Superior lot-to-lot consistency
- Extremely high specificity and sensitivity
- Recombinant rabbit monoclonal antibody
AS160 (AKT substrate of 160kDa), commonly known as TBC1D4 [(TBC1(tre-2/USP6,BUB2,cdc16) domain family member 4] is a 160kDa protein expressed in many tissues including brain, kidney, and liver. AS160 is a Rab GTPase activating protein (GAP) which regulates GLUT4 translocation by insulin-stimulated phosphorylation (Ref 1-3). AS160 also possesses a calmodulin-binding domain (CBD) which increases the possibility of contraction-stimulated increases in Ca(2+)/calmodulin (Ref 4). In human, the gene encoding AS160 is located on the q arm of chromosome 13 (Ref 5).
Exceptional Consistency Saves You Time and Money
ABfinity™ antibodies are produced by transfection of mammalian cells with heavy and light chain antibody cDNAs. This provides you with the highest consistency between lots, eliminating the need to revalidate your assays for each lot.
High Specificity Means More Reliable Data
The proprietary recombinant technology employed in the production of ABfinity™ antibodies means you are assured of the greatest degree of reliability. Staining of nonspecific proteins is virtually eliminated while very high sensitivity is achieved. Invitrogen has validated this antibody in Western blotting.
High Sensitivity Lets You Detect Low Levels of Target Protein
ABfinity™ antibodies demonstrate much higher sensitivity than ordinary antibodies. Proteins expressed in low levels can be detected with high specificity allowing you to use less of your precious samples than with ordinary antibodies.
Recombinant Rabbit IgG—Use Like Any Other IgG Antibody
As with traditional IgG antibodies, gel electrophoresis of ABfinity™ antibodies produces a 150 kDa band under non-reducing conditions and 50 kDa and 25 kDa bands under reducing conditions.
For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.
Related Links:
Learn more about the superior performance of ABfinity™ recombinant monoclonal antibodies.
See Invitrogen's full line of ABfinity™ recombinant monoclonal antibodies.
References:
- Kramer HF, Witczak CA, Taylor EB, Fujii N, Hirshman MF, Goodyear LJ. AS160 regulates insulin- and contraction-stimulated glucose uptake in mouse skeletal muscle. J Biol Chem. 2006 Oct 20; 281 (42):31478-85. PubMed PMID: 16935857.
- Baus D, Heermeier K, De Hoop M, Metz-Weidmann C, Gassenhuber J, Dittrich W, Welte S, Tennagels N. Identification of a novel AS160 splice variant that regulates GLUT4 translocation and glucose-uptake in rat muscle cells. Cell Signal. 2008 Dec; 20 (12):2237-46. PubMed PMID: 18771725.
- Ishibashi K, Kanno E, Itoh T, Fukuda M. Identification and characterization of a novel Tre-2/Bub2/Cdc16 (TBC) protein that possesses Rab3A-GAP activity. Genes Cells. 2009 Jan; 14 (1):41-52. PubMed PMID: 1907703.
- Kramer HF, Taylor EB, Witczak CA, Fujii N, Hirshman MF, Goodyear LJ. Calmodulin-binding domain of AS160 regulates contraction- but not insulin-stimulated glucose uptake in skeletal muscle. Diabetes. 2007 Dec; 56 (12):2854-62. PubMed PMID: 17717281.
- Kurihara LJ, Semenova E, Miller W, Ingram RS, Guan XJ, Tilghman SM. Candidate genes required for embryonic development: a comparative analysis of distal mouse chromosome 14 and human chromosome 13q22. Genomics. 2002 Feb; 79(2):154-61. PubMed PMID: 11829485.
